Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelationElectronic supplementary information (ESI) available: experimental details. See http://www.rsc.org/suppdata/cc/b2/b206054j/
- 5 August 2002
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 17,p. 1950-1951
- https://doi.org/10.1039/b206054j
Abstract
Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 °C increase in Tm of the complex.Keywords
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