Regional Quantitative Determination of Lactate in Brain Sections. A Bioluminescent Approach
Open Access
- 1 December 1985
- journal article
- research article
- Published by SAGE Publications in Journal of Cerebral Blood Flow & Metabolism
- Vol. 5 (4) , 609-612
- https://doi.org/10.1038/jcbfm.1985.90
Abstract
Regional lactate distribution in brain was assessed quantitatively in coronal sections using a bioluminescent technique. This bioluminescence can be induced by covering freeze-dried and heat-inactivated brain sections with a frozen solution containing enzymes and coenzymes both for lactate-dependent NADH formation and NADH-dependent bioluminescence, which was recorded photographically. Quantification and density coding of bioluminescent images were carried out by utilizing the regression coefficients of the correlation between the optical density of bioluminescent pictures and the lactate content measured in tissue samples. Regional quantitative lactate images were obtained from brain tissue taken from brain tumors or after experimental cerebral ischemia.Keywords
This publication has 18 references indexed in Scilit:
- Multiparametric Imaging of Blood Flow and Metabolism after Middle Cerebral Artery Occlusion in CatsJournal of Cerebral Blood Flow & Metabolism, 1985
- Regional changes of blood flow, glucose, and ATP content determined on brain sections during a single passage of spreading depression in rat brain cortexExperimental Neurology, 1984
- Metabolic Changes in Cerebral Cortex, Hippocampus, and Cerebellum During Sustained Bicuculline‐Induced SeizuresJournal of Neurochemistry, 1981
- Brain Lactic Acidosis and Ischemic Cell Damage: 1. Biochemistry and NeurophysiologyJournal of Cerebral Blood Flow & Metabolism, 1981
- A Bioluminescence Method for the Demonstration of Regional Glucose Distribution in Brain SlicesJournal of Neurochemistry, 1981
- Deleterious effect of glucose pretreatment on recovery from diffuse cerebral ischemia in the cat. II. Regional metabolite levels.Stroke, 1980
- Deleterious effect of glucose pretreatment on recovery from diffuse cerebral ischemia in the cat. I. Local cerebral blood flow and glucose utilization.Stroke, 1980
- Recovery of brain mitochondrial function in the rat after complete and incomplete cerebral ischemia.Stroke, 1979
- In vivo concentration of lactate in the brain of conscious rats before and during seizures: a new ultra‐rapid technique for the freeze‐sampling of brain tissueJournal of Neurochemistry, 1973
- SOME METABOLIC CHANGES ACCOMPANYING LEÃO'S SPREADING CORTICAL DEPRESSION IN THE RATJournal of Neurochemistry, 1961