Stimulation of the Differential Rate of Exoenzyme Formation in Bacillus Amyloliquefaciens by Streptolydigin, an Inhibitor of RNA Chain Elongation

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Abstract
Organism. The aerobic spore-forming bacillus used in this work was Bacillus amylolique- faciens strain T (Welker & Campbell, 1967). Growth conditions. The composition of the medium was: (NH,),HPO,, 34 mM; KCl, 5 mM; MgSO,, I mM; FeCl,, 0.5 mM; CaCl,, 0.125 mM; ZnSO,, 0.0125 mM; sodium citrate, 2.125 mM; 'trace metal ion' solution (Coleman & Elliott, 1969, 0.25 ml/l; vitamin solution, 50 ml/l; a mixture of equal amounts (by weight) of 19 naturally-occurring L-amino acids (cysteine omitted) to give a total amino acid concentration of 0-5 %; maltose, I % (w/v). The medium was adjusted to pH 7-2 with 10 % (v/v) phosphoric acid. The vitamin solution consisted of 0-1 mg riboflavin, and 1.0 mg each of inositol, biotin, choline chloride, folic acid, nicotinamide, calcium pantothenate, pyridoxal-5-phosphate and thiamine hydrochlo- ride in I 1 water. Batches (50 ml), contained in 250 ml conical flasks, were loop-inoculated from an aqueous spore suspension. The cultures were incubated at 30 "C in a gyrotory incubator-shaker (Model G25, New Brunswick Scientific Co., New Brunswick, New Jersey, U. S. A.) . Washed bacterial suspension experiments. Late exponential phase cultures (20 h) were centrifuged at 5000 g for 2 min and the supernatant fraction was discarded. The bacterial
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