The Effects of Actinomycin D, Puromycin, Cycloheximide and Hydroxyurea on 3′,5,3-Triiodo-L-thyronine Stimulated 2-Deoxy-D-glucose Uptake in Chick Embryo Heart Cellsin Vitro*†
- 1 July 1977
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 101 (1) , 150-156
- https://doi.org/10.1210/endo-101-1-150
Abstract
The uptake of 2-deoxy-D-1-3H-glucose (1-3H-DOG) by cultured chick embryo heart cells was stimulated by L-triiodothyronine (T3). This stimulation began with the exposure to T3, rose linearly for about 8 h and reached a plateau around 24 h. Actinomycin D, puromycin and cycloheximide had no effect on the stimulated sugar uptake during the first 6 h. These agents prevented the further increase in the rate of 1-3H-DOG uptake in T3-treated cultures that occurred between 6-24 h. In the presence of hydroxyurea the uptake of 1-3H-DOG in both control and T3-treated cells was unchanged. Some of the effects of T3 may not require new synthesis of either RNA, proteins or DNA.This publication has 3 references indexed in Scilit:
- The Effect of Triiodothyronine on 2-Deoxy-D-[l-3H]Glucose Uptake in Cultured Chick Embryo Heart Cells*Endocrinology, 1977
- Ribonucleic acid synthesis during the early action of thyroid hormonesBiochemical Journal, 1966
- Histological and Histochemical Uses of Periodic AcidStain Technology, 1948