Comparative Oxidative Metabolism of Aflatoxin B1and Palmotoxins B0and G0by Rat Liver Microsomal Fractions

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Abstract
1. The enzymic transformations of aflatoxin B1 and palmotoxins B0 and G0 by cell fractions of rat liver have been studied. Metabolism involves NADPH-dependent enzymes with max. activity in the presence of nicotinamide, MgCl2, glucose-6-phosphate and NADP. 2. The substances all undergo demethylation with production of formaldehyde. With both aflatoxin B1 and palmotoxin B0, two metabolites were detected by t.l.c. The two metabolites of aflatoxin B1 correspond to aflatoxin M1 and possibly aflatoxin B2a. The two derivatives of palmotoxin B0 have not been characterized but, like those of aflatoxin B1, are probably hydroxylation products, as they are more polar than the parent compound. Only one derivative of palmotoxin G0 was detected. 3. Aflatoxin B1 showed a higher rate of metabolism than the two other substances.