Three-dimensional models of human and other mammalian microsomal P450s constructed from an alignment with P450102 (P450bm3)
- 1 January 1995
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 25 (4) , 333-366
- https://doi.org/10.3109/00498259509061857
Abstract
1. A novel modelling alignment for P450s, utilizing NADPH-P450 reductase for electron transfer, is proposed on the basis of analysis of their amino acid sequences. 2. Information used to facilitate the alignment process includes: the recent X-ray crystal structure of P450102 (P450bm3), site-directed mutagenesis experiments, chemical modification of specific residues, and antibody recognition studies. 3. The alignment has been used to construct a number of microsomal P450s of relevance to xenobiotic and endogenous metabolism.Keywords
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