Enhancement of the polycation‐mediated DNA uptake and cell transfection with Pluronic P85 block copolymer
- 8 July 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 389 (3) , 278-280
- https://doi.org/10.1016/0014-5793(96)00607-2
Abstract
Polyelectrolyte complexes formed between DNA and poly(N-ethyl-4-vinylpyridinium) cations were shown to effectively transfect mammalian cells [7]. This work suggests that the polycation-mediated uptake of the plasmid DNA and cell transfection are significantly enhanced when these complexes are administered simultaneously with a poly(ethylene oxide)block-poly(propylene oxide)-block-poly(ethylene oxide) copolymer, Pluronic P85. The uptake studies were performed using radioactively labeled pRSV CAT plasmid on NIH 3T3, MDCK, and Jurkat cell lines. The transfection was investigated by chloramphenicol acetyltransferase assay using 3T3 cells as a model. The effects reported may be useful for the enhancement of the polycation-mediated cell transfection.Keywords
This publication has 11 references indexed in Scilit:
- Hypersensitization of Multidrug Resistant Human Ovarian Carcinoma Cells by Pluronic P85 Block CopolymerBioconjugate Chemistry, 1996
- DNA Complexes with Polycations for the Delivery of Genetic Material into CellsBioconjugate Chemistry, 1995
- An Evaluation of Receptor‐Mediated Gene Transfer Using Synthetic DNA‐Ligand ComplexesEuropean Journal of Biochemistry, 1994
- Gene Transfer with Synthetic Cationic Amphiphiles: Prospects for Gene TherapyBioconjugate Chemistry, 1994
- Gene transfer in vivo: sustained expression and regulation of genes introduced into the liver by receptor-targeted uptake.Proceedings of the National Academy of Sciences, 1994
- Efficient transformation of mammalian cells using DNA interpolyelectrolyte complexes with carbon chain polycationsBioconjugate Chemistry, 1993
- A new class of drug carriers: micelles of poly(oxyethylene)-poly(oxypropylene) block copolymers as microcontainers for drug targeting from blood in brainJournal of Controlled Release, 1992
- The neuroleptic activity of haloperidol increases after its solubilization in surfactant micellesFEBS Letters, 1989
- Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.Molecular and Cellular Biology, 1982
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976