OPTIMUM CULTURAL CONDITIONS FOR STRONG LIGHT PRODUCTION BY PHOTOBACTERIUM PHOSPHOREUM

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Abstract
Strong light intensity of the culture broth and industrially feasible cultural conditions are required in order to apply the bioluminescence of luminous bacteria to attracting fish. To obtain strong bioluminescence of the culture broth, studies of the optimum cultural conditions for strong light production by P. phosphoreum MT-10201 were carried out. Strong light intensity of the culture broth was obtained at an initial NaCl concentration in the medium between 3-5.5%, starting pH 8.5, and cultivation temperature 20.degree. C. The addition of 0.1% malic acid to the medium resulted in enhanced light intensity of the culture broth but mannose, arginine, dodecylaldehyde and phosphate buffer used for pH control during cultivation had no effect. Cultivation of the luminous bacterium in 2-l and 20-l fermenters was carried out, and luminous raw bait was prepared successfully by using the culture broth directly as a luminous source.

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