2′-MODIFIED OLIGONUCLEOTIDES FROM METHOXYOXALAMIDO AND SUCCINIMIDO PRECURSORS: SYNTHESIS, PROPERTIES, AND APPLICATIONS

31 March 2001

journal article
Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids

Vol. 20 (4-7) , 507-514
https://doi.org/10.1081/ncn-100002326

Abstract

Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) and 2'-succinimido (SUC) precursors is described. Their physical and biochemical properties were assessed. Synthesized oligonucleotides were used as primers in advanced DNA sequencing protocols. An example of sequencing directly off genomic DNA template without prior cloning or PCR amplification is presented.

Keywords

MODIFIED OLIGONUCLEOTIDES
METHOXYOXALAMIDO
DNA SEQUENCING
APPLICATIONS SYNTHESIS
BIOCHEMICAL PROPERTIES
DNA TEMPLATE
SEQUENCING PROTOCOLS
SUCCINIMIDO
SEQUENCING DIRECTLY

This publication has 1 reference indexed in Scilit:

Synthesis of functionally modified oligonucleotides from methoxyoxalamido precursors
Tetrahedron Letters, 1996