FLOW-CYTOMETRY STUDIES OF INTRACELLULAR ADRIAMYCIN IN SINGLE CELLS-INVITRO

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (9) , 3489-3494
Abstract
Flow cytometry techniques were used to quantify the intracellular fluorescence of adriamycin [an antitumor agent] in living cells [mouse L-929 fibroblasts and Chinese hamster lung fibroblast V99-171]. Additionally, fluoresence-activated cell sorting was utilized to investigate the relationship between intracellular drug concentration and cell viability. Uptake of adriamycin in cultured cells was highly dependent upon the method of cell growth (e.g., suspension vs. monolayer cultures) and on cell density or cell crowding. With constant exposure conditions direct proportionality was observed between mean cellular fluorescence and external adriamycin concentration. Total cellular fluorescence increased more rapidly than nuclear fluorescence, although both reached an apparent equilibrium in several hours. Under well-controlled exposure conditions, mean cell survival correlated well with mean cellular adriamycin fluorescence. Similar results were observed for adriamycin effects on DNA synthesis. Considerable heterogeneity of cellular adriamycin levels was, however, observed in all cell populations, and fluorescence-activated cell sorting indicated that the cells with the least intracellular adriamycin did indeed survive best.