Developmental Regulation of Synaptotagmin I, II, III, and IV mRNAs in the Rat CNS
Open Access
- 15 February 1997
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 17 (4) , 1206-1216
- https://doi.org/10.1523/jneurosci.17-04-01206.1997
Abstract
Synaptotagmin I is an abundant synaptic vesicle protein that has an essential function in mediating Ca2+-triggered neurotransmitter release. We have analyzed the distribution of four neural synaptotagmin isoforms during postnatal development of the rat CNS by in situ hybridization. Synaptotagmin I, II, III, and IV genes have distinct patterns of spatiotemporal expression except in cerebellum granule cells, where the four transcripts were detected during the formation of parallel fiber/Purkinje cell synapses. Throughout development synaptotagmin I mRNAs were widely expressed in brain, whereas synaptotagmin II transcripts were predominant in spinal cord. At all stages synaptotagmin III mRNAs were expressed uniformly in most neurons examined, although at a low level. Synaptotagmin I, II, and III gene expressions mainly increased during development and persisted in adulthood, mirroring neuronal differentiation. Conversely, synaptotagmin IV transcripts were predominant during perinatal development in a heterogeneous population of neurons and subsequently were expressed uniformly at a low level. Intense labeling was observed in the hippocampal CA3 field and in the subiculum, but not in the CA1 field, of the newborn rat. In cerebral cortex, lamina-specific labeling was detected with a high expression in cell layer V. Only a small number of Purkinje cell clusters were labeled in the flocculus and paraflocculus of the cerebellum. Heterogeneous sets of neurons expressing synaptotagmin IV gene also were observed in spinal cord. We thus speculate that synaptotagmin IV may a play a role in the development of the mammalian nervous system.Keywords
This publication has 40 references indexed in Scilit:
- Interaction of SNARE Complexes with P/Q-type Calcium Channels in Rat Cerebellar SynaptosomesPublished by Elsevier ,1996
- Differential distributions of novel synaptotagmins: Comparison to synapsinsNeuropharmacology, 1995
- Ca2+-dependent and -independent activities of neural and non-neural synaptotagminsNature, 1995
- Synaptotagmin controls and modulates synaptic-vesicle fusion in a Ca2+-dependent mannerTrends in Neurosciences, 1995
- Synaptotagmin V: a novel synaptotagmin isoform expressed in rat brainFEBS Letters, 1995
- Synaptotagmin I is a high affinity receptor for clathrin AP-2: Implications for membrane recyclingCell, 1994
- Synaptophysin expression during synaptogenesis in the rat cerebellar cortexJournal of Comparative Neurology, 1989
- Development of the hippocampal region in the rat I. Neurogenesis examined with 3H‐thymidine autoradiographyJournal of Comparative Neurology, 1980
- Evidence for a multiple innervation of purkinje cells by climbing fibers in the immature rat cerebellumJournal of Neurobiology, 1976
- Postnatal development of the cerebellar cortex in the rat. III. Maturation of the components of the granular layerJournal of Comparative Neurology, 1972