An improved high performance liquid chromatographic post‐column derivatization procedure for the UV — Vis spectroscopic characterization of phenolic compounds
- 1 February 1991
- journal article
- research article
- Published by Wiley in Phytochemical Analysis
- Vol. 2 (1) , 38-42
- https://doi.org/10.1002/pca.2800020108
Abstract
An improved method is described for the characterization of phenolic compounds using post‐column shift reagents following their separation by high performance liquid chromatography (HPLC). Modifications include the use of NaOH to adjust the pH of the column effluent, prior to introducing the AICI3 and H3BO3 reagents, and the use of commercially available spin chambers for efficiently mixing the effluent with the reagents, resulting in a negligible loss of resolution. Shifts obtained by the HPLC post‐column derivatization and the conventional procedure are comparable.Keywords
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