Immunocytochemical localization of tubulin, actin, and myosin in axonemes of ciliated cells from quail oviduct.
Open Access
- 1 May 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (10) , 3198-3202
- https://doi.org/10.1073/pnas.79.10.3198
Abstract
Tubulin, actin, and myosin have been localized in isolated demembranated ciliated cells from quail oviduct by immunocytochemistry in both light and electron microscopy by using purified antibodies. The peripheral doublets and the central tubules are stained by the antitubulin whereas the kinetosomes are poorly stained. Actin antibodies clearly stain the axonemes, but only on the proximal-half portion, whereas myosin antibodies stain a small area of the axonemes just above the ciliary neck region.Keywords
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