HO-323, a human B-lymphoblastoid cell line useful for making human-human hybridomas

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Abstract
A 6-thioguanine-resistant human B-lymphoblastoid cell line, Ho-323, was isolated for making human-human hybridomas with high efficiency. Fusions with peripheral blood lymphocytes of systemic lupus erythematosus (SLE) patients and lymphocytes isolated from lymph modes of lung and breast cancer patients yield constantly more than one hybridoma clones per 105 HO-323 cells plated. HO323 cells also fused with lymphocytes from normal peripheral blood to give hybridomas in the same efficiency. The HO-323 cells were diploid with 46 chromosomes and non-secretors of immunoglobulins. These parent cells doubled every 15 hr and could proliferate in serum-containing medium even if they were plated at low cell density of less than 103 cells/ml. The cells could grow in serum-free medium as well as in serum-containing medium, and the resultant human-human hybridomas could also grow in the same media.