Polynucleotides. LVI1. Synthesis and properties of poly(2′-deoxy-2′-fluoroinosinic acid)
- 1 January 1978
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (9) , 3315-3324
- https://doi.org/10.1093/nar/5.9.3315
Abstract
Poly (2'-deoxy-2'-fluoroinosinic acid) [ poly(If)] was synthesized by polymerization of 2'-deoxy-2'-fluoroinosine 5'-diphosphate catalyzed by Escherichia coli polynucleotide phosphorylase. Although the UV absorption properties of poly(If) closely resembled those of poly(I), thermal melting curves at Na+ concentrations of 0.15M and 0.75M suggested two ordered structures for poly(If) neutral form. CD psectra taken at 0.15M Na+ concentration showed rather larger amplitudes in both a peak at 273 nm and a trough at 246 nm, suggesting rather strong vertical stacking of bases. When complexed with poly(C), poly(If) forms a double-stranded complex, poly(If).poly(C) which has Tm's higher by 10-20 degrees than those of poly(If).poly(C) measured under the same conditions. The CD spectrum of this complex resembled that of poly(I).poly(C). The effect of the fluorine atom at the 2'-position on thermal stability of polynucleotides is discussed.Keywords
This publication has 13 references indexed in Scilit:
- Polynucleotides. LII.1Synthesis and properties of poly(2′-deoxy-2′-fluoroadenylic acid)Nucleic Acids Research, 1978
- Polynucleotides. L. Synthesis and properties of poly (2′ - chloro - 2′ - deoxyadenylic acid) and poly (2′ - bromo - 2′ - deoxyadenylic acid)Nucleic Acids Research, 1977
- Polynucleotides. XLV1)Synthesis and properties of poly(2′-azido-2′-deoxyinosinic acid)Nucleic Acids Research, 1977
- Poly-2′-deoxy-2′-fluoro-cytidylic acid: enzymatic synthesis, spectroscopic characterization and interaction with poly-inosinic acidNucleic Acids Research, 1977
- Polynucleotides. XLVI.1Synthesis and properties of poly (2′-amino-2′-deoxyadenylic acid)Nucleic Acids Research, 1977
- Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acidNucleic Acids Research, 1976
- Structures for polyinosinic acid and polyguanylic acidBiochemical Journal, 1974
- Synthesis and properties of poly 2′-fluoro-2′-deoxyuridylic acidBiochemical and Biophysical Research Communications, 1972
- Physical and chemical characterization of the ordered complexes formed between polyinosinic acid, polycytidylic acid and their deoxyribo-analoguesJournal of Molecular Biology, 1965
- The molecular structure of polyinosinic acidBiochimica et Biophysica Acta, 1958