Distribution of AMP‐Deaminase Isozymes in Rat Tissues

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Abstract
The distribution of AMP deaminase [EC 3.5.4.6] isozymes in rat tissues was analyzed by electrophoresis on cellulose acetate membrane, by chromatography on phosphocellulose column and by the application of immunological technique employing specific antisera against 3 parental AMP deaminases (isozymes A, B and C). Skeletal muscle extracts and diaphragm extracts contain a single identical isozyme isozyme A. The major isozyme species of liver, kidney and testes are also identical and they are isozyme B. Heart extracts contain isozyme C exclusively. Extracts of brain, lung and spleen contain 5 isozymes, presumably a complete set of 5 B-C hybrids. Developmental patterns of AMP deaminase isozyme were studied. In early postnatal life, extracts of heart, liver, kidney and lung contain 5 isozymes similar to those observed in adult brain. During postnatal development, a shift to isozyme C occurs in the heart, whereas a shift to isozyme B occurs in the liver and kidney. Five isozymes in the lung remain throughout development. In the brain a shift of B to 5 isozymes is observed during development. Isozyme A is the predominant form in the muscle throughout postnatal development. AMP deaminase in the regenerating liver was analyzed, but the data indicated that there was no change of isozyme distribution during hepatic regeneration.