Rubella-specific IgM detection using Sephacryl S-300 gel filtration.
Open Access
- 1 November 1980
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 33 (11) , 1082-1085
- https://doi.org/10.1136/jcp.33.11.1082
Abstract
Sephacryl S-300 has been compared with Sephadex G-200 in the fractionation of sera for the detection of rubella-specific IgM. No difference in sensitivity was found with sera that had low titres of rubella-specific IgM. Sephadex G-200 was apparently more sensitive with some sera which had high titres of rubella-specific IgM due to the contribution of rubella-specific IgA. Sephacryl S-300 offers considerable advantages in ease and speed of preparation, and as it is a more rigid gel higher flow rates can be obtained. Sera may be fractionated in 2.5 hours on Sephacryl S-300 compared with 7-10 hours on Sephadex G-200.This publication has 5 references indexed in Scilit:
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