Brief Communication: Reversible Loss in Suspension Culture of a Major Cell-Surface Glycoprotein of the TA3-Ha Mouse Tumor23

Publisher Website
Google Scholar
Abstract
A major cell-surface glycoprotein of the TA3-Ha ascites mammary adenocarcinoma diminished during transfer from ascites growth to cell growth in suspension culture. A sensitive, hemagglutination—inhibition assay that used a lectin from Vicia graminea seeds indicated approximately a 50% loss after 7–10 days of culture and a 90% loss after 2 months. These findings were corroborated by carbohydrate and amino acid analysis with gas-liquid chromatography of trypsin glycopeptides released from the cell surface. Repassage of the cultured cells in vivo caused the reappearance of the surface glycoprotein.