Determination of Methylenomycin A Synthesis by the pSV1 Plasmid from Streptomyces violaceus-ruber SANK 95570
- 1 August 1982
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 128 (8) , 1893-1901
- https://doi.org/10.1099/00221287-128-8-1893
Abstract
A plasmid (pSV1) of 110 .times. 106 daltons from a methylenomycin A producing strain of S. violaceus-ruber was detected on and isolated from agarose gels. Elimination of this plasmid by protoplasting and regeneration resulted in the simultaneous loss of methylenomycin A production and resistance. Plasmid pSV1 hybridized with pBR322 containing a cloned fragment of 1.7 .times. 106 daltons from S. coelicolor A3(2) which codes for methylenomycin A resistance. The pSV1 plasmid could be transferred to S. lividans by conjugation and by transformation and plasmid DNA identical in size to pSV1 could be isolated from the recipient strains. Plasmid pSV1 evidently codes for methylenomycin A production and resistance, in close analogy to the SCP1 plasmid from S. coelicolor A3(2).This publication has 10 references indexed in Scilit:
- Identification and location of l-glycerate, an unusual acyl substituent in gellan gumCarbohydrate Research, 1986
- Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividansMolecular Genetics and Genomics, 1981
- Linkage of genes for nitrogenase and nodulation ability on plasmids in Rhizobium leguminosarum and R. phaseoliMolecular Genetics and Genomics, 1981
- A rapid and efficient procedure for the purification of DNA from agarose gelsAnalytical Biochemistry, 1980
- A DNA cloning system for interspecies gene transfer in antibiotic-producing StreptomycesNature, 1980
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6Molecular Genetics and Genomics, 1978
- Genetic Determination of Methylenomycin Synthesis by the SCP1 Plasmid of Streptomyces coelicolor A3(2)Microbiology, 1977
- Identification of the Antibiotic Determined by the SCP1 Plasmid of Streptomyces coelicolor A3 (2)Journal of General Microbiology, 1976
- Genetic Studies on SCP1-prime Strains of Streptomyces coelicolor A 3 (2)Journal of General Microbiology, 1976