Searching Sequence Databases Over the Internet: Protein Identification Using MS‐Fit
Open Access
- 1 May 1998
- journal article
- unit
- Published by Wiley in Current Protocols in Protein Science
- Vol. 14 (1) , 16.5.1-16.5.6
- https://doi.org/10.1002/0471140864.ps1605s14
Abstract
Peptide fingerprint mass mapping is widely used for initial identification of proteins separated by gel electrophoresis. This method involves the enzymatic in-gel digestion of proteins to generate peptides, followed by mass measurement of the cleaved peptides. The experimental mass values are then compared with theoretical values from protein databases, calculated using the cleavage specificity of the enzyme. This unit describes the use of the search program MS-Fit to identify the closest match or matches.Keywords
This publication has 9 references indexed in Scilit:
- Peptide Sequencing by Mass Spectrometry for Homology Searches and Cloning of GenesProtein Journal, 1997
- Conserved Motifs as the Basis for Recognition of Homologous Proteins Across Species Boundaries Using Peptide-mass FingerprintingJournal of Mass Spectrometry, 1997
- Protein identification from 2-DE gels by MALDI mass spectrometryMass Spectrometry Reviews, 1997
- Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gelsProceedings of the National Academy of Sciences, 1996
- Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass measurement accuracy by using delayed extractionJournal of the American Society for Mass Spectrometry, 1996
- Delayed Extraction Improves Specificity in Database Searches by Matrix-assisted Laser Desorption/Ionization Peptide MapsRapid Communications in Mass Spectrometry, 1996
- Mass Spectrometry in the Biological SciencesPublished by Springer Nature ,1996
- Delayed extraction matrix‐assisted laser desorption time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 1995
- Rapid identification of proteins by peptide-mass fingerprintingCurrent Biology, 1993