Effect of dexamethasone on parathyroid hormone stimulation of cyclic AMP in an opossum kidney cell line
- 1 September 1987
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 132 (3) , 517-523
- https://doi.org/10.1002/jcp.1041320314
Abstract
The influence of the glucocorticoid dexamethasone on the cAMP response to parathyroid hormone (PTH) and various agonists was studied in epithelial monolayers of opossum kidney (OK) cells. The incubation with dexamethasone for 72 hours led to a dose-dependent higher cAMP response to PTH or forskolin in intact cells as well as in digitonin-permeabilized cells. This effect did not appear to result from changes in phosphodiesterase (PDE) activity nor from alterations in cAMP efflux from the cells. Moreover, dexamethasone increased the formation of domes by OK cell epithelium. Thus, dexamethasone seems to promote a more differentiated renal epithelial phenotype as suggested by enhanced hormonal response.Keywords
This publication has 33 references indexed in Scilit:
- Heterologous desensitization by 1,25-dihydroxyvitamin D-3 of cyclic AMP response to parathyroid hormone in osteoblast-like cells and the role of the stimulatory guanine nucleotide regulatory proteinBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Regulation of β-Adrenergic Receptors by Steroid HormonesAnnual Review of Physiology, 1984
- Guanine nucleotide-binding regulatory proteins and dual control of adenylate cyclase.Journal of Clinical Investigation, 1984
- Cytosolic activator of adenylate cyclase: Reconstitution, characterization, and mechanism of action.Proceedings of the National Academy of Sciences, 1983
- Stimulation of dome formation in MDCK kidney epithelial cultures by inducers of differentiation: Dissociation from effects on transepithelial resistance and cyclic AMP levelsJournal of Cellular Physiology, 1982
- Glucocorticoids increase the Na+-H+ exchange and decrease the Na+ gradient-dependent phosphate-uptake systems in renal brush border membrane vesicles.Proceedings of the National Academy of Sciences, 1982
- Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukaemiaNature, 1978
- An Effect of Dexamethasone on Adenosine 3′,5′ -Monophosphate Content and Adenosine 3′,5′ -Monophosphate Phosphodiesterase Activity of Cultured Hepatoma CellsJournal of Clinical Investigation, 1972