Ki‐ras Activation in Pancreatic Carcinomas of Syrian Hamsters Induced by N‐Nitrosobis(2‐hydroxypropyl)amine
Open Access
- 1 September 1991
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 82 (9) , 965-968
- https://doi.org/10.1111/j.1349-7006.1991.tb01928.x
Abstract
Five pancreatic carcinomas induced by N‐nitrosobis(2‐hydroxypropyl)amine in Syrian golden hamsters were analyzed for activation of Ki‐ras at codons 12 and 13, using the polymerase chain reaction and direct sequencing. The Ki‐ras gene was shown to be activated in four of the five carcinomas, and the results were further confirmed by subcloning and sequencing. All the mutations involved a G‐to‐A transition at the second position of codon 12, which resulted in a change at the amino acid level from glycine to aspartic acid. This mutation is identical with that reported for pancreatic tumors of Syrian hamsters induced by N‐nitrosobis(2‐oxopropyl)amine.Keywords
This publication has 25 references indexed in Scilit:
- Activation of K-ras in transplantable pancreatic ductal adenocarcinomas of Syrian golden hamstersCarcinogenesis: Integrative Cancer Research, 1990
- Usefulness of rapid production model for pancreatic carcinoma in male hamstersCancer Letters, 1990
- Frequent Glycine‐to‐Aspartic Acid Mutations at Codon 12 of c‐Ki‐ras Gene in Human Pancreatic Cancer in JapaneseJapanese Journal of Cancer Research, 1990
- Pancreatic ductal adenocarcinomas induced in syrian hamsters by N‐nitrosobis (2‐oxopropyl) amine contain a c‐Ki‐ras oncogene with a point‐mutated codon 12Molecular Carcinogenesis, 1990
- Activation of c‐Ki‐ras not detectable in adenomas or adenocarcinomas arising in rat pancreasMolecular Carcinogenesis, 1990
- Frequency and Types of Point Mutation at the 12th Codon of the c‐Ki‐ras Gene Found in Pancreatic Cancers from Japanese PatientsJapanese Journal of Cancer Research, 1989
- High frequency of Ki‐ras codon 12 mutations in pancreatic adenocarcinomasInternational Journal of Cancer, 1989
- Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genesPublished by Elsevier ,1988
- Animal models of exocrine pancreatic carcinogenesisCancer and Metastasis Reviews, 1987
- Establishment of a human pancreatic adenocarcinoma cell line (PSN-1) with amplifications of both c-myc and activated c-Ki-ras by a point mutationBiochemical and Biophysical Research Communications, 1986