EFFECT OF HYDROSTATIC PRESSURE ON THE ULTRASTRUCTURE OF ESCHERICHIA COLI

Abstract
The effect of pressure on the morphology and ultrastructure of E. coli IAM 1996 with particular reference to filament formation is studied. The cells were grown in a nutrient broth supplemented with 1.0% glucose and incubated at 25.degree. C for 60 h at ambient or increased pressure of 400 atm. By EM, the cells grown under high pressure exhibited cell elongation with a decrease in septum formation. More than 70% of the cells under high pressure exceeded 20-50 .mu.m in length, with some longer than 100-200 .mu.m as compared with 2-4 .mu.m for normal cells. Cytoplasmic dense granulations in the abnormal cells were also observed.

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