Cell-free Synthesis of a Specific Lipoprotein of the Escherichia coli Outer Membrane Directed by Purified Messenger RNA
Open Access
- 1 October 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (10) , 4149-4153
- https://doi.org/10.1073/pnas.71.10.4149
Abstract
A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.Keywords
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