Differentiation between Bacterial and Tryptic Gelatin Liquefaction as Aid to Diagnosis of Fibrosis of Pancreas

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Abstract
Certain bacteria, not rarely found in the feces of infants, may produce X-ray film gelatin liquefaction indistinguishable from that caused by pancreatic trypsin. It is desirable, therefore, to be able to distinguish between these enzymes, if the X-ray film test with fecal specimens for the laboratory diagnosis of cystic fibrosis of the pancreas be made more reliable. Soybean trypsin inhibitor, which prevents the liquefaction of gelatin(X-ray film) by crystalline trypsin and trypsin in feces, fails to inhibit gelatin liquefaction by various bacteria, including Proteus ammoniae, P. morgana, and Pseudomonas aeruginosa. Suitable preparations of ZnO, ZnO2, and Na perborate have little or no effect on trypsin in feces but markedly inhibit the bacterial enzyme responsible for gelatin liquefaction.
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