Effect of ammonium chloride on the multiplication of infectious pancreatic necrosis virus

Abstract
The multiplication of IPNV in CHSE cells is inhibited by ammonium chloride. This inhibition is complete if NH4Cl is added early in the infective cycle. Immunofluorescence analysis shows that NH4Cl reduces the number of virus producing cells and thus suggests it may act by inhibiting virus internalization. In addition, NH4Cl can produce partial inhibition of IPNV multiplication if added later in the replicative cycle. Analysis of3H-uridine incorporation into viral RNA shows that this later effect could be an inhibition of viral RNA synthesis.