Spermatozoa of the Atlantic bottlenosed dolphin, Tursiops truncatus

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Abstract
Semen from a male dolphin in captivity was collected by electro-ejaculation and frozen to -176.degree. C. Sperm motility was excellent after thawing 10 days later. EM showed 14-16 parallel ridges in the post-acrosomal region and 2 types of mitochondria in the midpiece. The spermatozoa were capable of fusing with zona-free hamster eggs only after preincubation for 2 h, suggesting the need for sperm capacitation and acrosome reaction before fertilization in this species.

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