The concentration of mammalian cells in a tangential flow filtration unit

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Abstract
A tangential flow filtration unit is described through which cell suspension is continuously recycled. A pressure drop is incurred across the filter which forces liquid through the filter sheet, and cells in the remaining suspension are thus concentrated. The unit described has been used to concentrate 4 1 of cell suspension containing at least 2 × 106 cells/ml 12‐to 23‐fold in 2 to 4 h, with 94% viable cell recovery and little loss of viability. The filter is amenable to scale‐up and a pilot scale version is under test in this laboratory.

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