Characterization of a ComE3 Homologue Essential for DNA Transformation in Helicobacter pylori
Open Access
- 1 September 2003
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (9) , 5427-5431
- https://doi.org/10.1128/iai.71.9.5427-5431.2003
Abstract
To find genes involved in natural competence in Helicobacter pylori, we used a bioinformatics database search and found two transformation-related open reading frames (ORFs): a comE3 homologue (HP1361 ORF) of Bacillus subtilis and a comL homologue (HP1378 ORF) of Neisseria gonorrhoeae. We failed to obtain an HP1378 ORF knockout mutant, while an HP1361 ORF knockout mutant was obtained by transposon shuttle mutagenesis. The DNA transformation abilities of both natural transformation and electroporation were severely impaired (frequency, −9) in the HP1361− mutant. Complementation with a pHel2 vector carrying the HP1361 ORF restored the capabilities of natural competence (to a frequency of 4.21 × 10−7) and electroporation (to 3.62 × 10−7). The HP1361− mutant showed impairment in DNA binding and uptake. The results suggest that HP1361 is a comE3 homologue and is required for DNA binding and uptake during DNA transformation.Keywords
This publication has 26 references indexed in Scilit:
- Association of Metronidazole Resistance and Natural Competence in Helicobacter pyloriAntimicrobial Agents and Chemotherapy, 2002
- Identification of Genes Associated with Natural Competence in Helicobacter pylori by Transposon Shuttle Random MutagenesisBiochemical and Biophysical Research Communications, 2001
- Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion systemMolecular Microbiology, 2001
- ComE, a Competence Protein from Neisseria gonorrhoeae with DNA-Binding ActivityJournal of Bacteriology, 2001
- Acid-Induced Gene Expression in Helicobacter pylori : Study in Genomic Scale by MicroarrayInfection and Immunity, 2001
- ThedprAgene is required for natural transformation ofHelicobacter pyloriFEMS Immunology & Medical Microbiology, 2000
- Helicobacter pyloriPopulations in Peruvian PatientsClinical Infectious Diseases, 1997
- Characterization of comE, a late competence operon of Bacillus subtilis required for the binding and uptake of transforming DNAMolecular Microbiology, 1993
- Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance markerJournal of General Microbiology, 1993
- DNA UPTAKE IN HAEMOPHILUS TRANSFORMATIONAnnual Review of Genetics, 1982