Chromosomal location of genes controlling lactate dehydrogenase in rye, wheat, and barley
- 1 February 1992
- journal article
- Published by Canadian Science Publishing in Genome
- Vol. 35 (1) , 32-34
- https://doi.org/10.1139/g92-005
Abstract
The chromosomal location of lactate dehydrogenease (LDH) genes in rye, barley, and wheat was investigated using the disomic standard addition sets of 'Chinese Spring' – 'Imperial' (wheat–rye) and 'Chinese Spring' – 'Betzes (wheat–barley) and the monosomic rye–wheat additions, respectively. Gel electrophoresis was performed on 5% polyacrylamide gels. Diagnostic bands for LDH isozymes were present only in zone 2. Genes controlling LDH isozymes in rye were found to be located on rye chromosome 4R (Ldh-R1and Ldh-R2) and in barley on chromosome 4Hv (Ldh-H1 and Ldh-H2). Because of the incomplete rye–wheat addition set, only one gene for LDH isozymes of wheat could be located (wheat chromosome 4B; Ldh-B1). In the three cereal species investigated, LDH genes are located generally on homoeologous group 4 chromosomes.Key words: gene location, lactate dehydrogenase, Secale cereale, Triticum aestivum, Hordeum vulgare.Keywords
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