A Simple Radioenzymatic Method to Measure Picogram Amounts of DOPA in Brain and Biological Fluids
- 1 January 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 36 (1) , 290-292
- https://doi.org/10.1111/j.1471-4159.1981.tb02406.x
Abstract
A simple radioenzymatic method for the determination of DOPA is described. The method is based on the conversion of DOPA to 3-O-[methyl-3H]DOPA by catechol-O-methyltransferase in the presence of S-adenosyl-[methyl-3H]methionine and purification of the labelled product by Sephadex G10 and Dowex 50 W × 4 ion exchange resin. The method has been applied to the assay of endogenous DOPA in different brain areas and to measuring DOPA accumulation after inhibition of aromatic amino acid DOPA decarboxylase.Keywords
This publication has 8 references indexed in Scilit:
- RADIOENZYMATIC ASSAY OF FEMTOMOLE CONCENTRATIONS OF DOPA IN TISSUES AND BODY FLUIDSJournal of Neurochemistry, 1979
- Stress-induced increase in 3,4-dihydroxyphenylacetic acid (DOPAC) levels in the cerebral cortex and in n. accumbens: Reversal by diazepamLife Sciences, 1978
- RAPID CONCURRENT AUTOMATED FLUORIMETRIC ASSAY OF NORADRENALINE, DOPAMINE, 3, 4‐DIHYDROXYPHENYLACETIC ACID, HOMOVANILLIC ACID AND 3‐METHOXYTYRAMINE IN MILLIGRAM AMOUNTS OF NERVOUS TISSUE AFTER ISOLATION ON SEPHADEX G10Journal of Neurochemistry, 1977
- An enzymatic—isotopic method for DOPA and its use for the measurement of dopamine synthesis in rat substantia nigra 1Journal of Neurochemistry, 1976
- Inhibition of catechol-o-methyl transferase by catechols and polyphenolsBiochemical Pharmacology, 1973
- A method for the determination of 3,4-dihydroxyphenylalanine (DOPA) in brainNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1972
- Simultaneous measurement of tyrosine and tryptophan hydroxylase activities in brain in Vivo using an inhibitor of the aromatic amino acid decarboxylaseNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1972
- Enzymatic O-Methylation of Epinephrine and Other CatecholsJournal of Biological Chemistry, 1958