Imidazoline Receptor Antisera‐Selected cDNA Clone and mRNA Distributiona
- 1 June 1999
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 881 (1) , 1-7
- https://doi.org/10.1111/j.1749-6632.1999.tb09335.x
Abstract
A novel cDNA, designated Imidazoline Receptor Antisera-Selected cDNA-1 (iras-1), encodes a 167-kD protein. Two of its predicted peptides (42-43 kD) are immunologically consistent with a previously reported 1(1)-imidazoline binding protein. In the present study, two forms of iras mRNA (6.0 and 9.5 kb) were quantified across fresh rat tissues. Highest levels were found in brain (almost exclusively 6.0 kb in size), followed by liver and lung (9.5 > or = 6.0 kb iras mRNA), kidney (6.0 > 9.5 kb), heart (6.0 kb), spleen (6.0 > or = 9.5 kb), testes (6.0 > 9.5 kb), and skeletal muscle (6.0 > 9.5 kb). A correlation exists (p = 0.71, p = 0.05) between total (6.0 + 9.5 kb) iras mRNA and I1 BMAX values across rat tissues, corrected for housekeeping gene expression. Thus, total iras mRNA appears to be roughly proportional to the density of I1-imidazoline binding sites.Keywords
This publication has 7 references indexed in Scilit:
- Characterization of a partial cDNA clone detected by imidazoline receptor-selective antiseraJournal of the Autonomic Nervous System, 1998
- Co-detection by two imidazoline receptor protein antisera of a novel 85 kilodalton proteinBiochemical Pharmacology, 1998
- The elusive family of imidazoline binding sitesTrends in Pharmacological Sciences, 1996
- Molecular characterization and isolation of a 45-kilodalton imidazoline receptor protein from the rat brainMolecular Brain Research, 1995
- Antiidiotypic Antibodies as Tools to Study Imidazoline ReceptorsAnnals of the New York Academy of Sciences, 1995
- Optimization of Radioligand Binding Assays for I1‐Imidazoline Sitesfn1Annals of the New York Academy of Sciences, 1995
- Isolation of a human cerebral imidazoline-specific binding proteinEuropean Journal of Pharmacology, 1994