A New Transfer RNA Fragment Reaction: TpΨpCpGp Bound to a Ribosome-Messenger RNA Complex Induces the Synthesis of Guanosine Tetra- and Pentaphosphates
- 1 August 1974
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (8) , 3226-3229
- https://doi.org/10.1073/pnas.71.8.3226
Abstract
The tetranucleotide TpΨpCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The TpΨpCpGp-dependent fragment reaction has a strict requirement for the 50S and 30S ribosomal subunits and synthetic or natural messenger RNA.Keywords
This publication has 13 references indexed in Scilit:
- Nonribosomal Synthesis of Guanosine 5′,3′-Polyphosphates by the Ribosomal Wash of Stringent Escherichia coliProceedings of the National Academy of Sciences, 1973
- Synthesis of Guanosine Tetra- and Pentaphosphate Requires the Presence of a Codon-Specific, Uncharged Transfer Ribonucleic Acid in the Acceptor Site of RibosomesProceedings of the National Academy of Sciences, 1973
- Identification of the Synthesis of Guanosine Tetraphosphate (MS I) as Insertion of a Pyrophosphoryl Group into the 3′-Position in Guanosine 5′-DiphosphateProceedings of the National Academy of Sciences, 1973
- MSI and MSII made on Ribosome in Idling Step of Protein SynthesisNature, 1972
- The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response.1970
- Functional Sites of Transfer RNA for the Binding to Messenger RNA-Ribosome ComplexThe Journal of Biochemistry, 1970
- Separation of Large Quantities of Ribosomal Subunits by Zonal UltracentrifugationEuropean Journal of Biochemistry, 1970
- Ribosome-catalyzed peptidyl transfer: substrate specificity at the P-site.Proceedings of the National Academy of Sciences, 1968
- Rapid ion-exchange chromatographic microanalysis of ultraviolet-absorbing materials and its application to nucleosidesAnalytical Biochemistry, 1968
- Nucleotide Sequence of KB Cell 5 S RNAScience, 1967