Phosphoenolpyruvatcarboxykinase aus Phycomyces blakesleeanus Bgff. / Phosphoenolpyruvate Carboxykinase in Phycomyces blakesleeanus Bgff.
Open Access
- 1 October 1978
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 33 (9-10) , 667-670
- https://doi.org/10.1515/znc-1978-9-1011
Abstract
Phosphoenolpyruvate carboxykinase (PEPCK) from Phycomyces blakesleeanus was partially purified by protamine sulfate precipitation, ammoniumsulfate precipitation, and diethylamino ethyl cellulose (DEAE) treatment. This preparation was employed for the characterization of the enzyme. The Km values for phosphoenolpyruvate (PEP) and ADP were determined as 1.6 and 0.42 mᴍ. The nucleotid specifity was demonstrated for ADP exclusively. The use of sulfuryl reagents showed the presence of thiol groups sensitive against p-hydroxymercuribenzoate but not effected by N-ethylmaleimide.Keywords
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