Analysis of UV-induced DNA photoproducts by 32P-postlabelling
- 1 January 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 16 (1) , 113-118
- https://doi.org/10.1093/carcin/16.1.113
Abstract
UV-Induced cyclobutane dimers and 6–4 photoproducts, containing an unmodilled nucleotide at the 5'-posltion were released from DNA by means of digestion with DNase I, snake venom phosphodlesterase and prostatic acid phos phatase. The enzymes were deactivated by proteinase K followed by ethanol precipitation. The products were phos phorylated by polynucleotide kinase and [γ-32P]ATP. The TLC system used for the analysis enables separation of the different photoproducts and detection at a fmol level. T4 endonuclease treatment was applied to confirm the positions of cyclobutane dimers.Keywords
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