Kinetics of pump currents generated by the Na+,K+‐ATPase
- 16 November 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 224 (1) , 83-88
- https://doi.org/10.1016/0014-5793(87)80427-1
Abstract
Purified Na+,K+-ATPase from pig kidney was attached to black lipid membranes. Pump currents of the enzyme could be measured with a time resolution of approx. 1 ms by releasing ATP from caged ATP with a UV laser flash. Analysis of the transient currents shows that a slow non-electrogenic step is followed by an electrogenic transition with a rate constant of 100 s−1 (22°C). The exponential components found in the transient currents are compared to transitions in the Albers-Post schemeKeywords
This publication has 17 references indexed in Scilit:
- Effects of the ATP, ADP and inorganic phosphate on the transport rate of the Na+,K+-pumpBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- Na+ movement in a single turnover of the Na pump.Proceedings of the National Academy of Sciences, 1984
- Photocurrent kinetics of purple-membrane sheets bound to planar bilayer membranesThe Journal of Membrane Biology, 1981
- A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP.Proceedings of the National Academy of Sciences, 1980
- Photocurrents generated by bacteriorhodopsin on planar bilayer membranesEuropean Biophysics Journal, 1979
- Bovine brain Na+, K′-stimulated ATP phosphohydrolase studied by a rapid-mixing technique, K′-stimulated liberation of [32P]orthophosphate from [32P]phosphoenzyme and resolution of the dephosphorylation into two phasesBiochimica et Biophysica Acta (BBA) - Enzymology, 1975
- Purification and characterization of
Na K ATPase III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphateBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974 - Phosphorylation and dephosphorylation reactions of bovine brain (Na+K+)-stimulated ATP phosphohydrolase studied by a rapid-mixing techniqueBiochimica et Biophysica Acta (BBA) - Enzymology, 1974
- Flexibility of an Active Center in Sodium-Plus-Potassium Adenosine TriphosphataseThe Journal of general physiology, 1969
- Biochemical Aspects of Active TransportAnnual Review of Biochemistry, 1967