Construction of a Human Cytochrome c Gene and Its Functional Expression in Saccharomyces cerevisiae
- 1 June 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 103 (6) , 954-961
- https://doi.org/10.1093/oxfordjournals.jbchem.a122393
Abstract
The nucleotide sequences of a partial cDNA and three pseudogenes of human cytochrome c were determined. The complete nucleotide sequences which encode human cytochrome c were constructed on the basis of one of the pseudogenes by in vitro mutagenesis. The constructed human cytochrome c was functionally expressed in Saccharomyces cerevisiae. The recombinant human cytochrome c was purified and characterized.Keywords
This publication has 13 references indexed in Scilit:
- Evolution of cytochrome c genes and pseudogenesJournal of Molecular Evolution, 1986
- Processed pseudogenes for rat cytochrome c are preferentially derived from one of three alternate mRNAs.Molecular and Cellular Biology, 1984
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Nonallelic members of the cytochrome c multigene family of the rat may arise through different messenger RNAsCell, 1983
- Transformation of intact yeast cells treated with alkali cationsJournal of Bacteriology, 1983
- Structure of the Schizosaccharomyces pombe cytochrome c gene.Molecular and Cellular Biology, 1982
- Isolation and structure of a rat cytochrome c gene.Journal of Biological Chemistry, 1981
- Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1980
- Sequence of the gene for iso-l-cytochrome c in saccharomyces cerevisiaeCell, 1979
- HUMAN HEART CYTOCHROME C - CHYMOTRYPTIC PEPTIDES, TRYPTIC PEPTIDES, AND COMPLETE AMINO ACID SEQUENCE1963