Role of Alginate O Acetylation in Resistance of MucoidPseudomonas aeruginosato Opsonic Phagocytosis
Open Access
- 1 March 2001
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (3) , 1895-1901
- https://doi.org/10.1128/iai.69.3.1895-1901.2001
Abstract
Establishment and maintenance of chronic lung infections with mucoidPseudomonas aeruginosain patients with cystic fibrosis (CF) require that the bacteria avoid host defenses. Elaboration of the extracellular, O-acetylated mucoid exopolysaccharide, or alginate, is a major microbial factor in resistance to immune effectors. Here we show that O acetylation of alginate maximizes the resistance of mucoidP. aeruginosato antibody-independent opsonic killing and is the molecular basis for the resistance of mucoidP. aeruginosato normally nonopsonic but alginate-specific antibodies found in normal human sera and sera of infected CF patients. O acetylation of alginate appears to be critical forP. aeruginosaresistance to host immune effectors in CF patients.Keywords
This publication has 67 references indexed in Scilit:
- Study of Cerebral Energy Metabolism Using the Rat Hippocampal Slice PreparationMethods, 1999
- Mucoid conversion of Pseudomonas aeruginos by hydrogen peroxide: a mechanism for virulence activation in the cystic fibrosis lungMicrobiology, 1999
- Pseudomonas aeruginosa in cystic fibrosis: role of mucC in the regulation of alginate production and stress sensitivityMicrobiology, 1997
- Gender differences in cystic fibrosis: Pseudomonas aeruginosa infectionJournal of Clinical Epidemiology, 1995
- Conversion to Mucoidy in Pseudomonas aeruginosaNature Biotechnology, 1993
- Protection Against Mucoid Pseudomonas aeruginosa in Rodent Models of Endobronchial InfectionsScience, 1990
- Mucoid Pseudomonas aeruginosa in cystic fibrosis: mutations in the muc loci affect transcription of the algR and algD genes in response to environmental stimuliMolecular Microbiology, 1990
- Chemistry and biology of the alginate of mucoid strains of pseudomonas aeruginosa in cystic fibrosisMolecular Aspects of Medicine, 1988
- Opsonophagocytic Killing Antibody toPseudomonas aeruginosaMucoid Exopolysaccharide in Older Noncolonized Patients with Cystic FibrosisNew England Journal of Medicine, 1987
- Polyagglutinability Due to Loss of O‐Antigenic Determinants in Pseudomonas Aeruginosa Strains Isolated from Cystic Fibrosis PatientsActa Pathologica Microbiologica Scandinavica Series B: Microbiology, 1985