Sizes and concentrations of several type C oncornaviruses and bacteriophage T2 by the resistive-pulse technique

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Abstract
Viruses > 60 nm in diameter may be rapidly sized to a few nanometers in their natural hydrated state as they pass one by one through a single pore in a newly developed nanometer-particle analyzer based on the resistive-pulse technique of the Coulter Counter and the use of submicron-diameter pores made by the Nuclepore process. Size measurements for several type C oncornaviruses are: Rauscher murine leukemia, 122.3 .+-. 2 nm; simian sarcoma, 109.7 .+-. 3 nm; Mason-Pfizer monkey, 140.0 .+-. 2.5 nm; RD-114, 115 .+-. 5 nm; and feline leukemia, 127.4 .+-. 2 nm, relative to standard 109-nm latex spheres. The T2 bacteriophage has a volume of (5.10 .+-. 0.15) .times. 10-16 cm3. Concentrations of viruses near 109-1011/ml that are fairly clear of debris are routinely measurable in a few minutes to an accuracy near 15%. A lower practical count limit is near 5 .times. 107 viruses/ml.