Translocation of an SH2‐containing protein tyrosine phosphatase (SH‐PTP1) to the cytoskeleton of thrombin‐activated platelets
- 18 April 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 343 (1) , 89-93
- https://doi.org/10.1016/0014-5793(94)80613-6
Abstract
A significant protein tyrosine phosphatase (PTP) activity was found to be associated with the cytoskeleton of thrombin‐stimulated platelets. Translocation of the enzyme became maximal within 1‐2 min of thrombin stimulation and was suppressed by cytochalasin D or upon inhibition of aggregation. Immunoblotting as well as immunoprecipitation revealed that a PTP with two SH2 domains (SH‐PTP1) displayed the same behaviour, translocation to the cytoskeleton showing the same time course as that observed for pp60 c‐src . We conclude that SH‐PTP1 might represent a critical enzyme in the complex interplay between the various proteins regulating protein tyrosine phosphorylation in the cytoskeletal matrix.Keywords
This publication has 50 references indexed in Scilit:
- Involvement of GPIIb-IIIa on Human Platelets in Phosphotyrosine-Specific DephosphorylationBiochemical and Biophysical Research Communications, 1993
- Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets.The Journal of cell biology, 1992
- SH2 and SH3 domains: From structure to functionCell, 1992
- Protein Tyrosine Phosphatases: A Diverse Family of Intracellular and Transmembrane EnzymesScience, 1991
- Polyphosphoinositide synthesis in platelets stimulated with low concentrations of thrombin is enhanced before the activation of phospholipase CFEBS Letters, 1990
- Association of phosphatidylinositol kinase and phosphatidylinositol 4‐phosphate kinase activities with the cytoskeleton in human plateletsFEBS Letters, 1989
- Are polyphosphoinositides involved in platelet activation?Biochemical and Biophysical Research Communications, 1983
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970