Accessory pigment fluorescence for quantitation of photosynthetic microbial populations

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Abstract
The in vivo fluorescence of the primary accessory pigments in purple bacteria (carotenoids), green bacteria (bacteriochlorophyll), green algae (chlorophyll), and cyanobacteria (phycocyanin) was found to be a linear function of cell concentration over three to four orders of magnitude. The lowest cell concentrations detectable were 104 cells/ml for procaryotes and 103 cells/ml for eucaryotes.