Preliminary report of the production of callus, organogenesis and regeneration of jojoba(Simmondsia chinensis, Link, Schneid.) in tissue culture

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Abstract
Shoot tips, leaves, stems and cotyledons of Simmondsia chinensis were cultured on Murashige and Skoog (M&S) medium supplemented with 3% sucrose and various auxins and cytokinins. Callus was initiated on all expiants when an auxin (generally 10-5 M conc.) such as NAA, IAA or 2,4-D, and a cytokinini (generally 10"5 M conc.) such as IP A, kinetin or zeatin, were added to the medium. Roots were induced on cultured leaf stem and cotyledon pieces. Shoot tips, which elongated in culture and branched laterally, were also induced to root. Rooting of elongated apices was accomplished by a two-step procedure involving different media, hormones and temperatures, resulting in complete plantlets. Nevertheless, plants developed from in vitro culture failed to survive when transferred in soil to the greenhouse.

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