Murine epidermal lipoxygenase (Aloxe) encodes a 12‐lipoxygenase isoform1
Open Access
- 14 January 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 402 (2-3) , 162-166
- https://doi.org/10.1016/s0014-5793(96)01517-7
Abstract
Using a combination of conventional screening procedures and polymerase chain reaction cloning, we have isolated a cDNA encoding an epidermis‐type 12‐lipoxygenase (e12‐lipoxygenase) from mouse epidermis. The open reading frame corresponds to a protein of 662 amino acids and was found to be 99.8% identical to the ORF of an epidermal lipoxygenase gene Aloxe, described recently [Van Dijk et al. (1995) Biochim. Biophys. Acta 1259, 4–8]. When expressed in human embryonic kidney cells the recombinant protein could be shown to synthesize 12(S)‐HETE from arachidonic acid. By fluorescence in situ hybridization the e12‐lipoxygenase gene was localized to chromosome band 11 B1–B3.Keywords
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