Demonstration of the presence of M-creatine kinase in mammalian myogenic cell lines
- 1 September 1978
- journal article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 34 (9) , 1149-1151
- https://doi.org/10.1007/bf01922924
Abstract
Unequivocal identification of M-CK in cell extracts from fused cells of myogenic cell lines is difficult due to almost identical behaviour of the M-CK and a contaminating enzyme activity in electrophoresis. If CK dimers present in cell extracts were subjected to dissociation and reassociation in the presence of exogenous B-CK subunits, the formation of easily identifiable MB-CK was demonstrated, indicating the presence of M-CK in the myogenic rat cell lines.Keywords
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