New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control
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- 19 April 2004
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (17) , 6421-6426
- https://doi.org/10.1073/pnas.0308014101
Abstract
The expression of certain genes involved in fundamental metabolism is regulated by metabolite-binding “riboswitch” elements embedded within their corresponding mRNAs. We have identified at least six additional elements within the Bacillus subtilis genome that exhibit characteristics of riboswitch function ( glmS , gcvT , ydaO/yuaA , ykkC/yxkD , ykoK , and yybP/ykoY ). These motifs exhibit extensive sequence and secondary-structure conservation among many bacterial species and occur upstream of related genes. The element located upstream of the glmS gene in Gram-positive organisms functions as a metabolite-dependent ribozyme that responds to glucosamine-6-phosphate. Other motifs form complex folded structures when transcribed as RNA molecules and carry intrinsic terminator structures. These findings indicate that riboswitches serve as a major genetic regulatory mechanism for the control of metabolic genes in many microbial species.Keywords
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