Herstellung von L‐Lysindecarboxylase aus Klebsiella pneumoniae

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Abstract
L‐Lysine decarboxylase (LL‐Lysine carboxylase E.C. 4.1.1.18) is produced by a strain of the genus Klebsiella.The bacterial cells are cultivated at a temperature of 37°C with and without shaking. The production of LL‐Lysine decarboxylase is increased by adding of LL‐Lysine to the medium. At the end of cultivation the cells are killed by thymol, washed in water and dried by means of acetone and ether.Compared with specimen derived from other bacteria the LL‐Lysine decarboxylase in the shape of dried cells shows a high specific activity. The preparations are free of disturbing enzyme activities, have a high level of stability and may be used for determining of LL‐Lysine by means of enzyme electrodes.