The replication origin of pSC101: Replication properties of a segment capable of autonomous replication.

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Abstract
One of 2 1.9 kb [kilobase] HaeII segments of pSC101 is capable of autonomous replication. Replication of a plasmid containing the segment retains characteristics of the pSC101-type replication such as the dnaA-dependence, the copy number of the plasmid, the location of the origin, the direction of replication and the incompatibility property. Analysis of deletion mutants revealed that a 1.65 kb region within the HaeII segment contained functions sufficient for autonomous replication. A terminal 0.6 kb segment on which the replication origin was mapped by an EM analysis of replicating molecules can replicate in the presence of a helper plasmid providing a trans-acting function and can express incompatibility toward pSC101.