Binding of Ferric Ions to Nuclei and Other Tissue Sites in Sections Stained for Sulfated Mucosubstances by the High-Iron Diamine Method

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Abstract
Binding of Fe3+ occurred in nuclei and several other sites when tissue sections, after a prior staining by the high-iron diamine (HID) method for sulfomucins, were immersed for 1 hr in 0.06 N HCl containing 1% potassium ferrocyanide (Prussian blue reaction). Apparently Fe3+, which is derived from FeCl3 present in the HID dye bath, unites directly with these tissue components, although one cannot exclude the possibility that iron is first bound to colorless diamine complexes and then to tissues. The visualization of Fe3+ by ferrocyanide provides a simple way of obtaining a suitable nuclear stain combined with general counters taming for the HID method.
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