pH on–off switching of antibody-hapten binding by site-specific chemical modification of tyrosine
Open Access
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection
- Vol. 7 (3) , 431-434
- https://doi.org/10.1093/protein/7.3.431
Abstract
Tetranitromethane (TNM) chemically mutates the binding sites of antibodies so that the nitrated antibodies exhibit pH-dependent binding near physiological pH. Three monoclonal antibodies were selectively modified, each under different conditions, with the resultant loss of binding activity at pH > 8 which is recovered at pH < 6. Recovery and loss of binding are ascribed to the protonation and deprotonation, respectively, of the hydroxyl group of the resulting 3-nitrotyrosine side chain (pKa∼ 7) at the binding site of these antibodies. pH on–off dependency of binding activity, common to all TNM-modified antibodies studied by us so far, may find use in a variety of applications in which controlled modulation under mild conditions is requiredKeywords
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