Demonstration Of Golgi Zones By Three Technics For Carbohydrates

Publisher Website
Google Scholar
Abstract
The carbohydrate of the Golgi apparatus of several organs of rats, rabbits, and frogs was selected as the principal test material for the behavior of three different technics: 1) periodic acid with colored fuchsin; 2) “direct” chromic acid piperazine silver; 3) periodic acid with leucofuchsin. Parallel sections of organs in which positive reactions were observed, were treated before staining with a series of reagents to characterize them as glycoprotein. The results obtained by the three technics under any constant set of conditions were essentially identical in all cases. It is concluded that discrepancies that may have been noted up to now are due to several factors, probably the most important being the tissue's physiological status and the influence of fixation. The study shows that HIO4, -fuchsinl and chromic acid silver methods are, at least empirically, as valid as HIO4, -leucofuchsin technics. Considering the differences in the oxidative mechanism of chromic and periodic acids and other data, the possibility of two different chemical pathways leading to the same final result is discussed. It has been found that colored fuchsin, as well as its leuco form, can be used in the histochemical demonstration of aldehydes after periodic acid treatment (Arzac, 1948). In a later report (Amc, 1950), a series of reactions were obtained with colored fuchsin which differed in several ways from the results of others using Hotchkiss' method. For example, Gersh (1949) reported the presence of probable glycoproteic granules in the Golgi apparatus of rabbit and guinea pig's intestine. Leblond (1950) also found positive Golgi reactions in different cells of male excretory ducts and in other organs of the rat. Such reactions had not been observed with the colored fuchsin technic in any of the two above-mentioned occasions. Since the latter investigators used different fixatives, which might have caused the discrepancies, the experiment described below was undertaken to study: (a) the influence of fixation on the final re-actions elicited by HI04-fuchsin (colored and leuco-form) and chromic acid piperazine silver methods; (b) the results obtained in the demonstration of Golgi zones of several rat's, rabbit's and frog's organs by these methods.

This publication has 5 references indexed in Scilit: